FITC labeled Lycopersicon Esculentum (Tomato) Lectin FITC標(biāo)記番茄凝集素
產(chǎn)品標(biāo)簽
番茄凝集素（Tomato lectin）；FITC-LEL；Intravascular perfusion血管內(nèi)灌注；Lectin perfusion 凝集素灌注；Microvasculature 微血管；Endothelial cells 內(nèi)皮細(xì)胞；
產(chǎn)品信息
產(chǎn)品名稱 | 產(chǎn)品編號 | 規(guī)格 | 目錄價（元） |
FITC labeled Lycopersicon Esculentum (Tomato) Lectin | MP6311-500UG | 500μg | 1700 |
產(chǎn)品描述
番茄凝集素（Tomato lectin，Lycopersicon Esculentum Lectin（LEL）），是一種非常穩(wěn)定的單亞基糖蛋白，約含有等量的蛋白質(zhì)和碳水化合物。據(jù)報道其糖類部分由85%阿拉伯糖和15%半乳糖組成，在溶液中可能形成多聚體聚合物。LEL無血型特異性，但能親和N-乙酰-β-D-葡糖胺寡聚體。LEL能夠很好的結(jié)合內(nèi)皮細(xì)胞，用來觀察和評價微血管系統(tǒng)。LEL能夠抑制菜豆來源植物血凝素（PMA）的促有絲分裂活性。
本品為FITC標(biāo)記的番茄凝集素（來源于Lycopersicon esculentum），Ex/Em=495/515nm，通過血管內(nèi)灌注法或直接染色組織切片能快速且有效的檢測和可視化嚙齒類動物的血管和小膠質(zhì)細(xì)胞。
產(chǎn)品特性
1） 英文同義名：Lycopersicon esculentum agglutinin（LEA），FITC Conjugate; Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin; FITC-LEL; FITC-LEA;
2） 分子量：~71kDa
3） 糖類特異性：[GlcNAc]1-3, N-Acetylglucosamine
4） F/P（摩爾）：2.0-5.0
5） 外觀：黃色至黃綠色液體（溶于10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca2+, 0.08% sodium azide and 5 mg/mL β-cyclodextrin）
6） 濃度：2mg/ml
7） Ex/Em=495/515nm
8） 應(yīng)用：糖生物；IF；
使用方法【源自文獻，僅作參考】
文獻1，Huang J et al. Regression of established tumors and metastases by potent vascular endothelial growth factor blockade. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7785-90. PMID: 12805568 體內(nèi)研究（Lectin Perfusion）： 動物模型（Animal Model）：Xenografts were established in 4- to 6-week-old female NCR nude mice by intrarenal injection of 106 SK-NEP-1 cultured human Wilms tumor cells and allowed to grow. After 5 weeks, large tumors were palpable in all mice, and a cohort was randomly selected to provide day-0 controls. 灌注方法（Lectin perfusion）：Selected mice at each time point underwent intravascular injection of fluorescein- labeled Lycopersicon esculentum lectin (100 μg in 100 μl of saline) into the left ventricle. The vasculature was fixed by infusion of 1% paraformaldehyde (pH 7.4) in PBS and then washed by perfusion of PBS. 給藥途徑（Administration）：Intravascular (i.v.) injection |
文獻2，Inai T et al. Inhibition of vascular endothelial growth factor (VEGF) signaling in cancer causes loss of endothelial fenestrations, regression of tumor vessels, and appearance of basement membrane ghosts. Am J Pathol. 2004 Jul;165(1):35-52. PMID: 15215160 體內(nèi)研究（Lectin Perfusion）： 動物模型（Animal Model）：Tumor-bearing RIP-Tag2 transgenic mice34 (C57BL/6 background, 10 to 12 weeks of age). 灌注方法（Lectin perfusion）：In mice used for immunohistochemistry, blood flow and patency of individual tumor vessels were assessed by injection of 100 μg of fluorescein isothiocyanate-labeled Lycopersicon esculentum lectin in 0.9% NaCl (100 μl into femoral vein). Two minutes later the chest was opened rapidly, and the vasculature was perfused for 2 minutes at a pressure of 120 mmHg with fixative [1% paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4] from an 18-gauge cannula inserted into the aorta via an incision in the left ventricle. Blood and fixative exited through an opening in the right atrium. Tissues were removed, immersed in fixative for 1 hour at 4°C, and then processed for immunohistochemistry. 給藥途徑（Administration）：Intravascular (i.v.) injection |
文獻3，Dawitz J et al. Functional calcium imaging in developing cortical networks. J Vis Exp. 2011 Oct 22;(56). pii: 3550. doi: 10.3791/3550. PMID: 22041662 體外研究： 細(xì)胞類型（Cell type）：Microglia and endothelial cells 實驗方法（Assay）：Take 25 μl of 2mg/ml Lycopersicon esculentum (tomato) lectin FITC conjugate stock solution into 2.5ml r-ACSF to give 20 μg/ml concentration. Pipette the dye over the slices as before, leaving the slices incubating for a period of 45 minutes. |
注意事項
1） 本品長期保存中可能產(chǎn)生沉淀，37℃溫育溶解后再使用。
2） 本品含防腐劑疊氮鈉，操作過程中請注意防護。
3） 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
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— —Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】
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FITC labeled Tomato Lectin 番茄凝集素
FITC labeled Tomato Lectin 番茄凝集素
