詳細介紹
產品名稱:TTC*
:021—60449724,
產品單位:gm
產品規格:5/10/25
CAS號:298-96-4
氯代三苯基四氮唑(triphenyltetrazolium chloride,TTC),也稱紅四氮唑(Red tetrazoline,RT)。可用來測定種子活力,被種子中的還原性物質(脫氫酶)還原為紅色的TTF,從而使活種子胚著上紅色而胚乳不被染色。而死 種子*不被染色。
1. 浸泡 將待測種子在 30 ~ 35 ℃溫水中浸種(大麥、小麥 6 小時,玉米 5 小時左右),以增強種胚的呼吸作用。 2. 顯色 取吸脹的種子 200 粒,用刀片沿種子胚的中心線縱切為兩半,將其中的一半置于 2 只培養皿中,每皿 100 個半粒,加入適量的 0.5% 溶液,以覆蓋種子為度。然后置于 30 ℃恒溫箱中 1 h 。觀察結果,凡胚被染為紅色的是活種子。 將另一半在沸水中煮 5 min 殺死胚,作同樣染色處理,作為對照觀察。 3. 計算活種子的百分率
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