產品新聞

背景資料

The secreted polypeptide noggin，encoded by the NOG gene，binds and inactivates members of the transforming growth factor-beta （TGF-beta） superfamily signaling proteins，such as bone morphogenetic protein-4 （BMP4）. By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily，noggin may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect，both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes，such as neural tube fusion and joint formation. Recently，several dominant human NOG mutations in unrelated families with proximal symphalangism （SYM1） and multiple synostoses syndrome （SYNS1） were identified； both SYM1 and SYNS1 have multiple joint fusion as their principal feature，and map to the same region （17q22） as NOG. All NOG mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of human noggin is highly homologous to that of Xenopus，rat and mouse.

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來源宿主

大腸桿菌中重組表達

溶解建議

提供的小鼠頭蛋白（mNoggin）產品為經0.2um孔徑過濾滅菌的白色凍干粉末；該凍干粉末是從含小鼠頭蛋白（mNoggin）的30% CH3CN，0.1% TFA溶液中過濾滅菌并凍干產生的.我們建議在打開蓋之前輕微離心使內容物至底部，加10mM HAc以配制工作儲存液到0.1-1.0 mg/mL，后繼實驗過程中可根據實驗要求進一步稀釋.

保存建議

小鼠頭蛋白（mNoggin）在室溫可保存3周，或置于-18℃凍存用于長期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存.請避免反復凍融.

其他

ProSpec 是一家全球著名細胞因子蛋白生產和研發的公司，具有20多年的生產經驗，專注于蛋白（重組及合成）生產研發。我們的核心產品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。  生產工藝和規模使其可以提供毫克到克級蛋白，價格優于同類公司。絕大部分產品是天然成熟型蛋白，而不含有標簽蛋白?？紤]到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產品為凍干粉，因此易于運輸和保存。