產品新聞

背景資料

The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction （Kd = 10-15 M-1） between a protein and its ligand。 One avidin binds four biotins。 The bond formation between biotin and avidin/streptavidin is very rapid and， once formed， is unaffected by pH， organic solvents and other denaturing agents。 Both avidin and streptavidin have essentially irreversible biotin-binding properties since bound biotin can only be released by denaturing the subunits of the proteins。 The tight and specific binding of biotin and its derivatives to various avidins has been extensively explored for a number of biological applications。

產品描述

The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution。 The assay employs HABA （4?hydroxyazobenzene-2-carboxylic acid）， a reagent that shows dramatic spectral changes when bound to avidin。 Biotin easily displaces HABA from the HABA/Avidin complex， resulting in a decrease of absorption at 500 nm。 The kit is best used to determine biotin concentration in the range from 2 to 16 礛。 The assay can be performed in a cuvette or microplate format。

產品特點

本Amplite 熒光法生物素定量檢測試劑盒產品，操作簡單，快速，更多詳細內容歡迎查看AAT Bioiesquest品牌 提供的英文產品說明書。

保存建議

對于 Amplite 熒光法生物素定量檢測試劑盒 產品，我們推薦使用常溫進行運輸。在您收到貨物后，我們建議在低于-15℃溫度下冷凍保存，要求儲存環境干燥并避免光照。產品有效期為6個月。

其他

美國AAT Bioquest Inc。（前身是ABD Bioquest，Inc。）是一家為從事生命科學研究、診斷研發及藥物開發的科學家研發、生產和銷售生物分析研究試劑和試劑盒的公司。公司致力于光譜學檢測領域，包括顯色、熒光和生物發光技術。AAT Bioquest的產品幫助 的科學家和生物醫藥研究者更好的了解生物化學，免疫學，細胞生物學和分子生物學等領域。作為AAT Bioquest Inc。的中國區域代理，艾美捷科技為中國客戶提供光譜學檢測技術并應用于生化、生理代謝和細胞分析領域的產品，包括顯色，熒光和發光技術等全系列解決方案。