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α肌動蛋白(α Actin)試劑盒

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北京方程生物公司經營銷售α肌動蛋白(α Actin)ELISA試劑盒,可以提供免費代測,送檢贈京東卡活動正在進行中,詳情咨詢銷售人員

詳細介紹

ELISA是以免疫學反應為基礎,將抗原、抗體體的特異性反應與酶對底物的高效催化作用相結合起來的一種敏感性很高的試驗技術。

α肌動蛋白(α Actin)試劑盒是北京方程生物公司的優勢產品,2017年買α肌動蛋白(α Actin)送好禮*活動火熱進行中......

 

α肌動蛋白(α Actin)試劑盒

OJ抗體/抗異亮氨酰tRNA合成酶抗體

 

Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.

 

α肌動蛋白(α Actin)elisa試劑盒回收率是反應待測物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標液1PPM,就是1毫克/升,而作出標準數據為0.99毫克/升,就是說你的回收率是99%,這個與真實成分有密切的關系,說明方法的準確度。比如水中總無機氯含量測定,樣品水中含有無機氯20mg/L,取100mL被測水樣品,加入0.1mL濃度為10mg/mL的含無機氯標準樣品,測定時忽略體積變化,如果測定出樣品中無機氯為2.98mg/L,則認為回收率為99%。

 

α肌動蛋白(α Actin)試劑盒

原鈣黏素1(PCDH1)

抗IVIgG抗體

*(PP)

Ⅳ型膠原(Col Ⅳ)

抗神經元核抗體2型/抗Ri抗體(ANNA-2/Ri)

乙胺碘呋酮(AD)

甘露聚糖結合凝集素相關*蛋白酶(MASP)

空泡毒素相關蛋白A(VacA)

B細胞淋巴瘤因子2(Bcl-2)

可溶性凋亡相關因子配體(sFASL)

Ⅰ型前膠原羧基端肽(PⅠCP)

抗組蛋白(H2A-H2B)-DNA抗體/抗二聚體-DNA抗體

 

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