試劑盒名稱:小鼠致癌基因相關蛋白KC ELISA試劑盒
Pig Trichohyalin (TCHH) ELISA
規格: 96T/48T
品牌:BIOFINE
種屬:人ELISA試劑盒
檢測波長:450nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃，六個月，-20℃一年
檢測目的:用于測定血清，血漿及相關液體小鼠致癌基因相關蛋白KC ELISA試劑盒含量。適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細胞培養上清、組織勻漿等標本。
ELISA實驗小知識：ELISA檢測試劑盒標本的稀釋原則：首先通過文獻檢索的方式了解待測樣本的大致含量，確定適當的稀釋倍數。只有稀釋至標準曲線的范圍內，檢測的結果才是準確的。稀釋的過程中，應做好詳細的記錄。zui后計算濃度時，稀釋了“N"倍，標本的濃度應再乘以“N"。
Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
人無翅型MMTV整合位點家族成員7B(WNT7B)測定盒Human Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) ELISA
人血小板衍生生長因子受體樣蛋白(PDGFRL)測定盒Human Plaet Derived Growth Factor Receptor Like Protein (PDGFRL) ELISA
豬Ⅰ型膠原(COL1)檢測盒Pig Collagen Type I (COL1) ELISA
豬腫瘤壞死因子α(TNFα)檢測盒Pig Tumor Necrosis Factor Alpha (TNFa) ELISA
豬神經元特異性烯醇化酶(NSE)檢測盒Pig Enolase, Neuron Specific (NSE) ELISA
豬多巴胺受體D4(DRD4)檢測盒Pig Dopamine Receptor D4 (DRD4) ELISA
豬胱天蛋白酶7(CASP7)檢測盒Pig Caspase 7 (CASP7) ELISA
豬視紫質(RHO)檢測盒Pig Rhodopsin (RHO) ELISA
豬絲裂原激活蛋白激酶12(MAPK12)檢測盒Pig Mitogen Activated Protein Kinase 12 (MAPK12) ELISA
豬無翅型MMTV整合位點家族成員5A(WNT5A)檢測盒Pig Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA
豬白蛋白(ALB)檢測盒Pig Albumin (ALB) ELISA
豬抗載脂蛋白抗體(AAHA)檢測盒Pig Anti-Apolipoprotein Antibodies (AAHA) ELISA
