培養基和添加劑 | RPMI-1640(GIBCO,貨號31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),0.05mM β-巰基乙醇,90%;優質胎牛血清,10%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。 |
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