培養基和添加劑 | DMEM培養基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，71.25%；F-12培養基(GIBCO,貨號21700075，添加L-glutamine 300mg/L, NaHCO3 1.5g/L)，23.75%；添加14mM HEPES；優質胎牛血清，5%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。 |
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