西班牙Certest單克隆抗體/單抗

廣州健侖生物科技有限公司

廣州健侖長期供應各種生物原料，主要代理品牌：西班牙Certest。

Certest Biotec，S.L. 是一家獨立的生物技術公司，致力于人類臨床領域的IVD診斷產品的開發和制造。快速檢測是基于快速、準確和易于操作的診斷產品。 此外，Cerstest還有多種實時PCR產品，可以用于醫院臨床、實驗室科研等。

Certest公司于2002年在薩拉戈薩成立，是一家創新技術型公司。公司的發展是基于新產品的開發、市場空間和機遇的探索，十幾年來一直以高度專業化，以客戶為導向，不斷得創新，優化產品，專業知識，取得廣大客戶的信任和支持。Certest的質量體系已通過ISO 13485認證。

主要產品包括各種生物單克隆抗原抗體、重組蛋白。

輪狀病毒單克隆抗體、腺病毒抗體、星狀病毒單克隆抗體、諾如病毒單克隆抗體、幽門螺旋桿菌抗體、隱球菌抗體、腸道病毒抗體、賈第鞭毛蟲抗體、彎曲桿菌抗體、阿米巴原蟲抗體、呼吸道合胞病毒單抗等等。

Certest公司 Certest單克隆抗體/單抗

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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西班牙Certest公司簡介

Certest Biotec, S.L. is an independent biotechnology company devoted to the development and manufacturing of IVD diagnostic products in human clinical field. Our Rapid Test are based in a fast, reliable and easy-interpretation process. In addition, VIASURE contains a wide range of products for Real Time PCR that allow to identify the causal pathogens of the infectious diseases in physicians’ offices, labs and hospitals.
The company was established in Zaragoza in 2002 as an innovative and technology-based company. The company’s growth is based on the development of new products and the exploration for new market niches and opportunities. The key to understand its success is the way to understand its organization: highly specialised and customer-oriented based.

Mission, Vision, Values and Human Team

Mission
To develop, produce and market a wide range of innovative solutions and quality in vitro diagnostics for the detection of viruses, bacteria and parasites.

Vision
Being the international leader company in research and development for in vitro diagnostic solutions, increasing our distribution network through added value creation and company-customer trust.

Values
Customer orientation · Ethical behaviour · Teamwork · Continuous research and innovation ·Trained and motivated team · Quality and creativity in all processes

Human team
The multi-skilled team is a dedicated and experience professional group responsible for dealing with all the different company activities in an international environment. 

Commitment to Innovation, Export and High-Technology.

Certest’s Quality System has been certified with ISO 13485.

One of the main objectives of Certest is to achieve customer satisfaction. For this, the management of the company ensures that customer requirements are determined and reviewed, identifying their needs and expectations, and translating them into requirements, all in order to maintain customer satisfaction.

See/Download the Certest Biotec ISO Standard Certification Certest Policy is consistent with its primary purpose, that of getting to all its products and services, quality and reliability, meeting the requirements and maintaining the effectiveness of the management system.

“Comparative study of two diagnostic tests for detection of RNA Zika Virus (ZIKV) in clinical samples”

“Comparison of Real-Time PCR test with the routine diagnosis technique to detect enteric pathogenic protozoa”

“Diagnostic of the Enteric Virus infection by Real-Time PCR in stool samples”

“Clinical performance evaluation of lyophilized VIASURE Real Time PCR Detection Kits for pathogen detection by participation in External Quality Assessment (EQA) programs”

“Detection and differentiation of Influenza A Virus, Influenza B Virus and H1N1 (pdm09) variant through an stabilized Real Time PCR Mix”

“Comparison of different molecular methods for the detection of non-influenza respiratory virus”virus”

【西班牙Certest生物原料】

Certest公司 

腺病毒對嚙齒類動物有致癌能力，或能轉化體外培養的嚙齒類動物細胞。使細胞轉化只需要腺病毒基因組的一部分，這些基因位于基因組的左端，約占整個基因組的7%～10%。盡管腺病毒分布很廣，但對人體不出現致癌性。人體細胞是一類允許細胞(permissive cell)，即這類細胞允許感染入侵的病毒在細胞內復制增殖，zui后細胞裂解死亡而釋放出大量子代病毒。在體外培養的多種人體腫瘤細胞中均未查出腺病毒顆粒，但在人的1號染色體上有adl2的整合位點，這意味著人體細胞對于腺病毒也可能是非允許細胞，即這類細胞在病毒感染后，病毒不能在細胞內復制增殖，但可整合在受感染細胞的基因組內。這些細胞被病毒轉化，表型發生改變，且可在體外無限期地培養傳代。
構成核酸檢測試劑盒
腺病毒呈無囊膜的球形結構,其病毒粒子在感染的細胞核內常呈晶格狀排列,每個病毒顆粒包含一個36 kb的線
腺病毒結構示意圖
腺病毒結構示意圖
性雙鏈DNA,兩端各有一個100~600 bp的反向末端重復序列(inverted terminal re-pea,t ITR), ITR的內側為病毒包裝信號,是病毒包裝所需要的順式作用元件。基因組包含早期表達的與腺病毒復制相關的E1~E4基因和晚期表達的與腺病毒顆粒組裝相關的L1~L5基因。線狀雙股DNA與核心蛋白形成直徑為60~65 nm的髓芯,被包裹于衣殼內。衣殼呈二十面體對稱,由252個直徑8~10 nm的殼粒組成,殼粒排列在三角形的面上,每邊6個,其中240個為六鄰體(非頂點殼粒) ,另12個為五鄰體基底(頂點殼粒)。每個六鄰體是六鄰體蛋白的同源三聚體,三聚體的六鄰體分子有一個三角形的塔尖和五面體的基底,塔區由4個環構成即loop1、loop2、loop3、loop4,基底包含兩個區域P1、P2區核酸檢測試劑盒。六鄰體上的表位(epitope)是診斷不同血清型的標準,它包括哺乳動物腺病毒屬的抗原成分,是病毒體對免疫選擇壓力zui敏感的部位。每個五鄰體基底上結合著1根(哺乳動物腺病毒)或2根(禽腺病毒)長9~77. 5 nm的纖維突起,這些纖維以五鄰體蛋白為基底由衣殼面伸出,纖維頂端形成頭節區,纖突有血清特異性,且含有負責體外血細胞凝集的種屬特異性抗原決定位點。核酸檢測試劑盒 
腺病毒含13%DNA和87%的蛋白質，病毒體分子量約為175×106。病毒基因組為線狀雙鏈DNA，大約含35kb～36kb，腺病毒12、18和31型的DNA組成中，G+C mol%zui低（48%～49%），屬于對動物具有高致癌性基因型。腺病毒1、2、4、5、8等型的G+C mol%較高（61%），致癌性反而低或無。這是一種用于人腺病毒分離株的分組的標準，根據其基因同源性將人腺病毒分為A～F等6組。
腺病毒的基因組以線性的雙鏈DNA形式存在，由蛋白VII和一種稱為mu的小蛋白緊密地環繞在其周圍，起到類組蛋白樣的作用。另一種蛋白V將這種DNA-蛋白復合物連接起來，并通過蛋白VI與病毒衣殼連接在一起。在兩條鏈的5′端各以共價鍵結合著一個被稱為DNA末端蛋白（pTP）復合物（DNA-TPC）的特化的結構，與腺病毒復制密切相關。腺病毒基因組的兩端各有一段100bp的反向末端重復序列（ITR），是復制的起始位點。在左端ITR的3′側有一段長約300bp的包裝信號（ψ）介導腺病毒基因組包裝入病毒衣殼。對腺病毒而言，只有包括兩端的ITR和包裝信號（ψ）的約0.5kb的序列是順式作用元件，也就是說必須由腺病毒載體自身攜帶，而其他的30余種蛋白都可以通過輔助病毒（或細胞）反式補足。

病毒蛋白約11種（TP和PⅠ～PⅩ），其中有4種蛋白（病毒多肽PⅤ、PⅦ，末端蛋白TP、酶蛋白PⅩ）與病毒基因構成病毒核心，多肽PⅦ是主要的核心蛋白，如同組蛋白一樣包裹病毒基因DNA。構成病毒衣殼的蛋白質約7種。多肽PⅡ是病毒衣殼中zui豐富和zui主要成分，六鄰體是由3個PⅡ分子緊密相連組成。多肽PⅥ、PⅧ在六鄰體與病毒核心之間形成連接橋，并與多肽PⅨ一起穩定著六鄰體分子的晶格排列。5個分子多肽PⅢ相連構成五鄰體的基座蛋白，PⅢa為五鄰體的周圍蛋白，也參與衣殼的組成，五鄰體通過PⅤ與病毒核心相連。多肽PⅣ主要構成病毒三聚體纖突，纖突與病毒血凝活性相關，因血凝素（纖突）具有型特異性，常用血凝抑制試驗（HI）對臨床分離株進行分型。

Certest公司 

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、食品安全、化妝品檢測、藥物濫用檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

想了解更多的產品及服務請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

Adenoviruses are carcinogenic to rodents or can transform rodent cells cultured in vitro. The transformation of cells requires only a portion of the adenoviral genome, which is located on the left side of the genome, accounting for about 7% to 10% of the entire genome. Although adenovirus is widely distributed, it does not appear carcinogenic to the human body. Human cells are a type of permissive cell, which allows the virus invaded to infect and proliferate in the cell. Finally, the cell lysis and death release large quantities of progeny virus. Adenovirus particles were not found in a variety of human tumor cells cultured in vitro, but there was an integration site for ad12 on human chromosome 1, which means that human cells may also be non-admissible cells for adenovirus, that is, After the virus is infected, the virus can not replicate and proliferate within the cell, but can integrate into the genome of the infected cell. These cells are transformed by the virus, the phenotype changes, and the passage can be cultured indefiniy in vitro.
Constitute a nucleic acid detection kit
Adenovirus showed a non-encapsulated globular structure, the virus particles in the infected nuclei often lattice-like arrangement, each virus particle contains a 36 kb line
Adenovirus structure diagram
Adenovirus structure diagram
Sex double-stranded DNA, each with a 100-600 bp inverted terminal re-pea (t ITR) at both ends, inside the ITR is a viral packaging signal and is a cis-acting element required for viral packaging. The genome contains E1 ~ E4 genes that are expressed early and adenoviral replication and L1 ~ L5 genes that are related to the assembly of adenovirus particles in the late stage. The linear double-stranded DNA and core protein form a core of 60-65 nm in diameter and are encapsulated within the capsid. The capsid is icosahedrally symmetrical and consists of 252 shell particles of 8-10 nm in diameter. The capsids are arranged on the surface of the triangle with 6 on each side, of which 240 are hexon (non-apex capsid) and the other Twelve were pentad bases (apex capsid). Each hexon is a homotrimer of hexon proteins. Hexamer molecules in the trimer have a triangular spire and a pentahedral base. The tower is composed of four loops, loop1, loop2, loop3 , Loop4, the substrate contains two regions P1, P2 nucleic acid detection kit. An epitope on a hexon is a standard for diagnosing different serotypes and includes the antigenic component of the mammalian adenovirus, which is the site of the virus most sensitive to immune selection pressure. Each penton base is bound to one (mammalian adenovirus) or two (avian adenoviruses) fibroblasts 9 to 77.5 nm in length that extend from the capsid surface on a penton protein basis , The top of the fiber forms the head region, the spike has serum specificity and contains the species-specific epitope responsible for in vitro hemagglutination. Nucleic acid test kit
The adenovirus contains 13% DNA and 87% protein, and the virion has a molecular weight of about 175 x 106. The viral genome is a linear double-stranded DNA, which contains about 35 kb to 36 kb of adenovirus DNA, and has the lowest G + C mol% (48% -49%) in the DNA of adenovirus type 12, 18 and 31, type. Adenovirus 1, 2, 4, 5, 8 and other types of G + C mol% higher (61%), carcinogenicity but low or no. This is a standard for grouping human adenovirus isolates. According to their gene homology, human adenovirus was divided into six groups, A to F,
The adenovirus genome exists as a linear, double-stranded DNA and acts as a histone-like protein by the protein VII and a small protein called mu, tightly surrounding it. Another protein V joins this DNA-protein complex and connects to the viral capsid via protein VI. At the 5 'end of both strands each covalently binds a specialized structure called the DNA terminal protein (DNA-TPC), which is closely related to adenovirus replication. The adenovirus genome has a 100bp inverted terminal repeat (ITR) at each end and is the origin of replication. A packaging signal of approximay 300 bp (ψ) on the 3 'side of the left ITR mediates the packaging of the adenovirus genome into the viral capsid. For the adenovirus, only about 0.5 kb of the sequence including the ITR at both ends and the packaging signal (ψ) is a cis-acting element, that is, it must be carried by the adenovirus vector itself and the other 30 proteins can pass Helper virus (or cell) trans complement.

There are about 11 kinds of virus proteins (TP and PⅠ ~ P Ⅹ). Among them, four proteins (viral peptides PⅤ, PⅦ, terminal protein TP and enzyme P X) form the virus core with the virus gene. The polypeptide PⅦ is the major core protein, Protein wraps virus DNA. About seven proteins that make up the viral capsid. Polypeptide PII is the most abundant and predominant component of the virus capsid, and the hexon is composed of three PII molecules closely linked. Peptides PVI and PⅧ form a bridging bridge between the hexon and the viral core and, together with the polypeptide PIX, stabilize the lattice arrangement of the hexon. Five molecules of peptide PIII are linked to form a pentaploid pep- tide protein. PIIIa is a pentagonal surrounding protein and is also involved in the composition of the capsid. The penton is linked to the viral core via PV. Peptide PIV mainly constitute the virus trimer fibrosis, fibroblasts and viral hemagglutination activity associated with hemagglutinin (fibroblasts) with type-specific, common hemagglutination inhibition test (HI) for clinical isolates.
Classification of nucleic acid detection kit
More than 100 serotypes have been found since the classification and classification of adenovirus isolated in the 1950s. Among them, there are 52 human adenoviruses, which are divided into six subtypes A, B, C, D, E and F Subgroup. Genes commonly used in human type 2 and 5 adenovirus in the serological classification are C subgroups, 95% homology in the DNA sequence. Both of them have very strong proliferative capacity. The titer can usually reach 109pfu (plaque forming unit) / ml, and its genome copy number can reach 104 (about 10% of the total cell DNA) in a single cell. The virus particles are relatively stable, which can reach 1010 ~ 1011pfu / ml by CsCl gradient centrifugation to meet the requirements of animal experiments.
Induced disease nucleic acid test kit
Adenovirus can infect the respiratory tract, gastrointestinal tract, urethra and bladder, eye, liver and the like. About 1/3 of known adenovirus serotypes are usually associated with human diseases, but one serotype can cause different clinical problems Conversely, different serotypes can cause the same condition.
Respiratory tract infections
Typical symptoms of respiratory infections are cough, stuffy nose and pharyngitis, accompanied by fever, chills, headache and muscle
Adenovirus
Adenovirus
Pain, etc., including the following four different syndromes.
1. Acute febrile throat is a common cause for infants and children and is caused by Group C viruses and presents with symptoms of cough, stuffy nose, fever and sore throat. These manifestations are difficult to differentiate from other mild respiratory infections caused by the virus.
2. Symptoms of pharyngoconjunctival fever are similar to acute febrile throat, but conjunctivitis is common. Pharyngeal conjunctival fever tendency outbreaks, such as swimming conjunctivitis, mostly caused by the B group of adenovirus type 3 and 7, the prognosis is good, generally no sequelae.
3. The syndrome of acute respiratory diseases (ARDs) is characterized by pharyngitis, fever, cough and general malaise. It is often prevalent in recruits of the military and is often caused by sudden stress, exertion, accumulation and the like. This infection caused by adenovirus 4,7 type, also found in type 3.
4, pneumonia adenovirus pneumonia accounts for about 10% of childhood pneumonia, mostly caused by adenovirus 3,7 type; adenovirus pneumonia in young people the mortality rate was 8% to 10%; pneumonia is also recruits a kind of acute respiratory disease Serious performance.
Eye infection
Adenovirus-induced eye infections are a complication of respiratory infections and pharyngolaryngitis. Follicular conjunctivitis can be caused by many adenoviruses, similar to chlamydial conjunctivitis, and is self limiting. Keratoconjunctivitis, caused by adenoviruses 8, 9 and 37, is a severe infection, highly contagious, starting with acute conjunctivitis and expanding to the anterior ear node, followed by keratitis.