產品英文名： Human procollagen Ⅱ N-terminal peptide,PⅡNP ELISA Kit ，人Ⅱ型前膠原N端前肽(PⅡNP)ELISA試劑盒市場常用名： 人Ⅱ型前膠原N端前肽(PⅡNP)ELISA檢測試劑盒
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elisa試劑盒總需要時間來保溫，是因為ELISA屬固相免疫測定，抗原、抗體的結合只在固相表面上發生。以抗體包被的夾心法為例，加入板孔中的標本，其中的抗原并不是都有均等的和固相抗結合的機會，只有zui貼近孔壁的一層溶液中的抗原直接與抗體接觸。這是一個逐步平衡的過程，因此需經擴散才能達到反應的終點。
在人Ⅱ型前膠原N端前肽(PⅡNP)ELISA試劑盒中一般有兩次抗原抗體反應，即加標本和加酶結合物后。抗原抗體反應的完成需要有一定的溫度和時間，這一保溫過程稱為溫育，有人稱之為孵育，在ELISA試劑盒中似不恰當。
Mus musculus (Mouse) 鈣非依賴性磷脂酶A2(iPLA2)酶聯免疫吸附試劑盒 ELISA Kit for Phospholipase A2, Calcium Independent (iPLA2) 48T/96T
Rattus norvegicus (Rat) 鈣非依賴性磷脂酶A2(iPLA2)酶聯免疫吸附試劑盒 ELISA Kit for Phospholipase A2, Calcium Independent (iPLA2) 48T/96T
Rattus norvegicus (Rat) 蛋白激酶Cβ1(PKCβ1)酶聯免疫吸附試劑盒 ELISA Kit for Protein Kinase C Beta 1 (PKCb1) 48T/96T
Canis familiaris; Canine (Dog) 骨形成蛋白1(BMP1)酶聯免疫吸附試劑盒 ELISA Kit for Bone Morphogenetic Protein 1 (BMP1) 48T/96T
Mus musculus (Mouse) 脂蛋白a(Lpa)酶聯免疫吸附試劑盒 ELISA Kit for Lipoprotein, a (Lpa) 48T/96T
Homo sapiens (Human) 雌激素受體β(ERβ)酶聯免疫吸附試劑盒 ELISA Kit for Estrogen Receptor Beta (ERb) 48T/96T
Rattus norvegicus (Rat) 對氧磷酶1(PON1)酶聯免疫吸附試劑盒 ELISA Kit for Paraoxonase 1 (PON1) 48T/96T
Rattus norvegicus (Rat) 膜鐵轉運蛋白(FPN)酶聯免疫吸附試劑盒 ELISA Kit for Ferroportin (FPN) 48T/96T
Homo sapiens (Human) 低密度脂蛋白受體相關蛋白2(LRP2)酶聯免疫吸附試劑盒 ELISA Kit for Low Density Lipoprotein Receptor Related Protein 2 (LRP2) 48T/96T
Mus musculus (Mouse) 生長停滯特異性蛋白6(GAS6)酶聯免疫吸附試劑盒 ELISA Kit for Growth Arrest Specific Protein 6 (GAS6) 48T/96T
Bos taurus; Bovine (Cattle) 突觸素(SYP)酶聯免疫吸附試劑盒 ELISA Kit for Synaptophysin (SYP) 48T/96T
Chicken (Gallus) 脂聯素(ADP)酶聯免疫吸附試劑盒 ELISA Kit for Adiponectin (ADP) 48T/96T
General 高香草酸(HVA)酶聯免疫吸附試劑盒 ELISA Kit for Homovanillic Acid (HVA) 48T/96T
Mus musculus (Mouse) 脂質運載蛋白1(LCN1)酶聯免疫吸附試劑盒 ELISA Kit for Lipocalin 1 (LCN1) 48T/96T
Rattus norvegicus (Rat) 脂質運載蛋白1(LCN1)酶聯免疫吸附試劑盒 ELISA Kit for Lipocalin 1 (LCN1) 48T/96T
Homo sapiens (Human) ⅩⅦ型膠原(COL17)酶聯免疫吸附試劑盒 ELISA Kit for Collagen Type XVII (COL17) 48T/96T
Homo sapiens (Human) *β10(TMSβ10)酶聯免疫吸附試劑盒 ELISA Kit for Thymosin Beta 10 (TMSb10) 48T/96T
Homo sapiens (Human) 胞漿抗蛋白水解酶3(CAP3)酶聯免疫吸附試劑盒 ELISA Kit for Cytoplasmic Antiproteinase 3 (CAP3) 48T/96T
Homo sapiens (Human) 肝配蛋白A1(EFNA1)酶聯免疫吸附試劑盒 ELISA Kit for Ephrin A1 (EFNA1) 48T/96T
Homo sapiens (Human) *S轉移酶θ1(GSTθ1)酶聯免疫吸附試劑盒 ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1) 48T/96T
Mus musculus (Mouse) *S轉移酶θ1(GSTθ1)酶聯免疫吸附試劑盒 ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1) 48T/96T
Rattus norvegicus (Rat) *S轉移酶θ1(GSTθ1)酶聯免疫吸附試劑盒 ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1) 48T/96T
General 脂多糖(LPS)酶聯免疫吸附試劑盒 ELISA Kit for Lipopolysaccharides (LPS) 48T/96T
Homo sapiens (Human) 胰島素樣生長因子結合蛋白5(IGFBP5)酶聯免疫吸附試劑盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 5 (IGFBP5) 48T/96T
Homo sapiens (Human) 趨化因子C-C-基元配體14(CCL14)酶聯免疫吸附試劑盒 ELISA Kit for Chemokine C-C-Motif Ligand 14 (CCL14) 48T/96T
Rattus norvegicus (Rat) 皮敵菌素(DCD)酶聯免疫吸附試劑盒 ELISA Kit for Dermcidin (DCD) 48T/96T
Homo sapiens (Human) *氨肽酶(AAP)酶聯免疫吸附試劑盒 ELISA Kit for Alanine Aminopeptidase (AAP) 48T/96T
Bos taurus; Bovine (Cattle) 催乳素(PRL)酶聯免疫吸附試劑盒 ELISA Kit for Prolactin (PRL) 48T/96T
Rattus norvegicus (Rat) Ⅰ型膠原α1(COL1α1)酶聯免疫吸附試劑盒 ELISA Kit for Collagen Type I Alpha 1 (COL1a1) 48T/96T
Homo sapiens (Human) 解整合素金屬蛋白酶17(ADAM17)酶聯免疫吸附試劑盒 ELISA Kit for A Disintegrin And Metalloprotease 17 (ADAM17) 48T/96T
Homo sapiens (Human) S100鈣結合蛋白A7(S100A7)酶聯免疫吸附試劑盒 ELISA Kit for S100 Calcium Binding Protein A7 (S100A7) 48T/96T
Bos taurus; Bovine (Cattle) 免疫球蛋白結合蛋白1(IGBP1)酶聯免疫吸附試劑盒 ELISA Kit for Immunoglobulin Binding Protein 1 (IGBP1) 48T/96T
Capra hircus; Caprine (Goat) 黃體激素(LH)酶聯免疫吸附試劑盒 ELISA Kit for Luteinizing Hormone (LH) 48T/96T
Mus musculus (Mouse) 微管關聯蛋白τ(MAPτ)酶聯免疫吸附試劑盒 ELISA Kit for Microtubule Associated Protein Tau (MAPt) 48T/96T
Rattus norvegicus (Rat) 微管關聯蛋白τ(MAPτ)酶聯免疫吸附試劑盒 ELISA Kit for Microtubule Associated Protein Tau (MAPt) 48T/96T
Mus musculus (Mouse) 血幼素(HJV)酶聯免疫吸附試劑盒 ELISA Kit for Hemojuvelin (HJV) 48T/96T
Homo sapiens (Human) Ⅱ型膠原α1(COL2α1)酶聯免疫吸附試劑盒 ELISA Kit for Collagen Type II Alpha 1 (COL2a1) 48T/96T
Mus musculus (Mouse) 乙醇脫氫酶1(ADH1)酶聯免疫吸附試劑盒 ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)人Ⅱ型前膠原N端前肽(PⅡNP)ELISA試劑盒 48T/96T
Mus musculus (Mouse) 線粒體乙醛脫氫酶(ALDM)酶聯免疫吸附試劑盒 ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM) 48T/96T
保溫的方式除有的ELISA儀器附有特制的電熱塊外，一般均采用水浴，可將ELISA試劑盒板置于水浴箱中，ELISA板底應貼著水面，使溫度迅速平衡。為避免蒸發，板上應加蓋，也可用塑料貼封紙或保鮮膜覆蓋板孔，此時可讓反應板漂浮在水面上。若用保溫箱，ELISA試劑盒應放在濕盒內，濕盒要選用傳熱性良好的材料如金屬等，在盒底墊濕的紗布，zui后將ELISA板放在濕紗布上。濕盒應先放在保溫箱中預溫至規定的溫度，特別是在氣溫較低的時候更應如此。無論是水浴還是濕盒溫育，反應板均不宜疊放，以保證各板的溫度都能迅速平衡。室溫溫育的反應，操作時的室溫應嚴格限制在規定的范圍內，標準室溫溫度是指20～25℃，但具體操作時可根據說明書的要求控制溫育。室溫溫育時，人Ⅱ型前膠原N端前肽(PⅡNP)ELISA試劑盒只要平置于操作臺上即可。應注意溫育的溫度和時間應按規定力求準確。為保證這一點，一個人操作時，一次不宜多于兩塊板同時測定。
