產品英文名：    Human 5-hydroxyeicosatetraenoic acid,5-HETE ELISA Kit    ，人5羥二十碳四烯酸(5-HETE)ELISA試劑盒市場常用名：    人5羥二十碳四烯酸(5-HETE)ELISA檢測試劑盒

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elisa試劑盒總需要時間來保溫，是因為ELISA屬固相免疫測定，抗原、抗體的結合只在固相表面上發生。以抗體包被的夾心法為例，加入板孔中的標本，其中的抗原并不是都有均等的和固相抗結合的機會，只有zui貼近孔壁的一層溶液中的抗原直接與抗體接觸。這是一個逐步平衡的過程，因此需經擴散才能達到反應的終點。
在人5羥二十碳四烯酸(5-HETE)ELISA試劑盒中一般有兩次抗原抗體反應，即加標本和加酶結合物后?？乖贵w反應的完成需要有一定的溫度和時間，這一保溫過程稱為溫育，有人稱之為孵育，在ELISA試劑盒中似不恰當。
Rattus norvegicus (Rat)    轉化生長因子β3(TGFβ3)酶聯免疫吸附試劑盒    ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)    48T/96T
Oryctolagus cuniculus (Rabbit)    轉化生長因子β誘導蛋白(TGFβI)酶聯免疫吸附試劑盒    ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)    48T/96T
Rhesus monkey (Simian)    轉化生長因子β3(TGFβ3)酶聯免疫吸附試劑盒    ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)    48T/96T
Bos taurus; Bovine (Cattle)    組織金屬蛋白酶抑制因子4(TIMP4)酶聯免疫吸附試劑盒    ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)    48T/96T
Sus scrofa; Porcine (Pig)    組織金屬蛋白酶抑制因子4(TIMP4)酶聯免疫吸附試劑盒    ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)    48T/96T
Oryctolagus cuniculus (Rabbit)    組織金屬蛋白酶抑制因子4(TIMP4)酶聯免疫吸附試劑盒    ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)    48T/96T
Capra hircus; Caprine (Goat)    腫瘤壞死因子α(TNFα)酶聯免疫吸附試劑盒    ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)    48T/96T
Equus caballus; Equine (Horse)    腫瘤壞死因子α(TNFα)酶聯免疫吸附試劑盒    ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)    48T/96T
Bos taurus; Bovine (Cattle)    腫瘤壞死因子β(TNFβ)酶聯免疫吸附試劑盒    ELISA Kit for Tumor Necrosis Factor Beta (TNFb)    48T/96T
Canis familiaris; Canine (Dog)    腫瘤壞死因子β(TNFβ)酶聯免疫吸附試劑盒    ELISA Kit for Tumor Necrosis Factor Beta (TNFb)    48T/96T
Sus scrofa; Porcine (Pig)    腫瘤壞死因子β(TNFβ)酶聯免疫吸附試劑盒    ELISA Kit for Tumor Necrosis Factor Beta (TNFb)    48T/96T
Oryctolagus cuniculus (Rabbit)    腫瘤壞死因子β(TNFβ)酶聯免疫吸附試劑盒    ELISA Kit for Tumor Necrosis Factor Beta (TNFb)    48T/96T
Rhesus monkey (Simian)    腫瘤壞死因子β(TNFβ)酶聯免疫吸附試劑盒    ELISA Kit for Tumor Necrosis Factor Beta (TNFb)    48T/96T
Bos taurus; Bovine (Cattle)    胰島素樣生長因子結合蛋白3(IGFBP3)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)    48T/96T
Capra hircus; Caprine (Goat)    胰島素樣生長因子結合蛋白3(IGFBP3)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)    48T/96T
Equus caballus; Equine (Horse)    胰島素樣生長因子結合蛋白3(IGFBP3)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)    48T/96T
Danio rerio (Zebrafish)    胰島素樣生長因子結合蛋白3(IGFBP3)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)    48T/96T
Chicken (Gallus)    胰島素樣生長因子結合蛋白3(IGFBP3)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)    48T/96T
Bos taurus; Bovine (Cattle)    胰島素樣生長因子結合蛋白4(IGFBP4)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)    48T/96T
Capra hircus; Caprine (Goat)    胰島素樣生長因子結合蛋白4(IGFBP4)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)    48T/96T
Chicken (Gallus)    胰島素樣生長因子結合蛋白4(IGFBP4)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)    48T/96T
Sus scrofa; Porcine (Pig)    胰島素樣生長因子結合蛋白4(IGFBP4)酶聯免疫吸附試劑盒    ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)    48T/96T
Bos taurus; Bovine (Cattle)    Ⅰ型膠原α2(COL1α2)酶聯免疫吸附試劑盒    ELISA Kit for Collagen Type I Alpha 2 (COL1a2)    48T/96T
Mus musculus (Mouse)    蛋白C(PROC)酶聯免疫吸附試劑盒    ELISA Kit for Protein C (PROC)    48T/96T
Rattus norvegicus (Rat)    蛋白C(PROC)酶聯免疫吸附試劑盒    ELISA Kit for Protein C (PROC)    48T/96T
Homo sapiens (Human)    Ⅳ型膠原α1(COL4α1)酶聯免疫吸附試劑盒    ELISA Kit for Collagen Type IV Alpha 1 (COL4a1)    48T/96T
Homo sapiens (Human)    外皮蛋白(EVPL)酶聯免疫吸附試劑盒    ELISA Kit for Envoplakin (EVPL)    48T/96T
Mus musculus (Mouse)    通用轉錄因子ⅡH肽1(GTF2H1)酶聯免疫吸附試劑盒    ELISA Kit for General Transcription Factor IIH, Polypeptide 1 (GTF2H1)    48T/96T
Canis familiaris; Canine (Dog)    單核細胞趨化蛋白4(MCP4)酶聯免疫吸附試劑盒    ELISA Kit for Monocyte Chemotactic Protein 4 (MCP4)    48T/96T
Equus caballus; Equine (Horse)    單核細胞趨化蛋白4(MCP4)酶聯免疫吸附試劑盒    ELISA Kit for Monocyte Chemotactic Protein 4 (MCP4)    48T/96T
Bos taurus; Bovine (Cattle)    *型纖溶酶原激活因子受體(uPAR)酶聯免疫吸附試劑盒    ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR)    48T/96T
Rhesus monkey (Simian)    *型纖溶酶原激活因子受體(uPAR)酶聯免疫吸附試劑盒    ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR)    48T/96T
Bos taurus; Bovine (Cattle)    血小板生成素(TPO)酶聯免疫吸附試劑盒    ELISA Kit for Thrombopoietin (TPO)    48T/96T
Canis familiaris; Canine (Dog)    血小板生成素(TPO)酶聯免疫吸附試劑盒    ELISA Kit for Thrombopoietin (TPO)    48T/96T
Chicken (Gallus)    血小板生成素(TPO)酶聯免疫吸附試劑盒    ELISA Kit for Thrombopoietin (TPO)    48T/96T
Sus scrofa; Porcine (Pig)    血小板生成素(TPO)酶聯免疫吸附試劑盒    ELISA Kit for Thrombopoietin (TPO)    48T/96T
Bos taurus; Bovine (Cattle)    *型纖溶酶原激活因子(uPA)酶聯免疫吸附試劑盒    ELISA Kit for Plasminogen Activator, Urokinase (uPA)    48T/96T
Chicken (Gallus)    *型纖溶酶原激活因子(uPA)酶聯免疫吸附試劑盒    ELISA Kit for Plasminogen Activator, Urokinase (uPA)    48T/96T
Sus scrofa; Porcine (Pig)    *型纖溶酶原激活因子(uPA)酶聯免疫吸附試劑盒    ELISA Kit for Plasminogen Activator, Urokinase (uPA)    48T/96T
Capra hircus; Caprine (Goat)    白介素10(IL10)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 10 (IL10)    48T/96T
Equus caballus; Equine (Horse)    白介素10(IL10)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 10 (IL10)    48T/96T
Bos taurus; Bovine (Cattle)    白介素12B(IL12B)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12B (IL12B)    48T/96T
Canis familiaris; Canine (Dog)    白介素12B(IL12B)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12B (IL12B)    48T/96T
Capra hircus; Caprine (Goat)    白介素12B(IL12B)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12B (IL12B)    48T/96T
Equus caballus; Equine (Horse)    白介素12B(IL12B)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12B (IL12B)    48T/96T
Danio rerio (Zebrafish)    白介素12B(IL12B)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12B (IL12B)    48T/96T
Cavia (Guinea pig )    白介素12B(IL12B)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12B (IL12B)    48T/96T
Bos taurus; Bovine (Cattle)    白介素12A(IL12A)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12A (IL12A)    48T/96T
Canis familiaris; Canine (Dog)    白介素12A(IL12A)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12A (IL12A)    48T/96T
Capra hircus; Caprine (Goat)    白介素12A(IL12A)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12A (IL12A)人5羥二十碳四烯酸(5-HETE)ELISA試劑盒    48T/96T
Equus caballus; Equine (Horse)    白介素12A(IL12A)酶聯免疫吸附試劑盒    ELISA Kit for Interleukin 12A (IL12A)    48T/96T

保溫的方式除有的ELISA儀器附有特制的電熱塊外，一般均采用水浴，可將ELISA試劑盒板置于水浴箱中，ELISA板底應貼著水面，使溫度迅速平衡。為避免蒸發，板上應加蓋，也可用塑料貼封紙或保鮮膜覆蓋板孔，此時可讓反應板漂浮在水面上。若用保溫箱，ELISA試劑盒應放在濕盒內，濕盒要選用傳熱性良好的材料如金屬等，在盒底墊濕的紗布，zui后將ELISA板放在濕紗布上。濕盒應先放在保溫箱中預溫至規定的溫度，特別是在氣溫較低的時候更應如此。無論是水浴還是濕盒溫育，反應板均不宜疊放，以保證各板的溫度都能迅速平衡。室溫溫育的反應，操作時的室溫應嚴格限制在規定的范圍內，標準室溫溫度是指20～25℃，但具體操作時可根據說明書的要求控制溫育。室溫溫育時，人5羥二十碳四烯酸(5-HETE)ELISA試劑盒只要平置于操作臺上即可。應注意溫育的溫度和時間應按規定力求準確。為保證這一點，一個人操作時，一次不宜多于兩塊板同時測定。