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小鼠血小板反應(yīng)蛋白/*敏感蛋白1<TSP-1>ELISA試劑盒國內(nèi)優(yōu)質(zhì)品牌，國外原裝品質(zhì)，多國品牌ELISA試劑盒，價格優(yōu)勢*，我們提供全程免費ELISA實驗代測，歡迎大家來免費觀摩代測實驗，相當(dāng)于培訓(xùn)一樣。
小鼠血小板反應(yīng)蛋白/*敏感蛋白1<TSP-1>ELISA試劑盒敏感性高、操作簡便，配套儀器設(shè)備的發(fā)展使操作程序規(guī)范化和自動化，并進(jìn)一步提高了穩(wěn)定性，被廣泛用于檢測多種病原體抗原或抗體、血液及其他體液中的微量蛋白成分、細(xì)胞因子等。ELISA應(yīng)用的范圍很廣，而且正在不斷地擴(kuò)大，原則上ELISA可用于檢測一切抗原、抗體及半抗原，可以直接定量測定體液中的可溶性抗原。
(1)檢查抗原和半抗原方面。尚有用于檢查大腸桿菌、銅綠假單胞菌、破傷風(fēng)梭菌毒素、輪狀病毒、呼吸道融合及巨細(xì)胞病毒等。在免疫化學(xué)方面可用于檢測瘋牛病、癢病等病原學(xué)檢測。
(2)檢查抗體方面。用ELISA間接法檢查抗體，已獲得對多種傳染病和寄生蟲病的血清學(xué)診斷，亦開始廣泛用于現(xiàn)場流行病學(xué)調(diào)查。在寄生蟲病方面，它用于對阿米巴、血吸蟲、肺吸蟲、肝吸蟲、旋毛蟲病等血清學(xué)診斷。在病原微生物方面已用于檢查鏈球菌、沙門菌、布氏桿菌、結(jié)核桿菌、流感病毒、輪狀病毒、皰疹病毒、狂犬病毒等，其敏感性都超過目前常用的檢查方法。
分類：ELISA常用的方法主要包括雙抗體夾心法、間接法、競爭法以及BAS-ELISA。
(1)雙抗體夾心法：雙抗體夾心法是檢測抗原zui常用的方法。夾心法利用兩種一抗對目標(biāo)抗原進(jìn)行捕獲和固定，在確保靈敏度的同時大大提高了反應(yīng)的特異性，該方法的檢測靈敏度可提高到pg級的水平。將已知抗體包被到固相表面，加入待檢標(biāo)本，標(biāo)本中若含有相應(yīng)抗原即與固相表面的抗體結(jié)合，洗滌去除未結(jié)合成分，加入該抗原特異的酶標(biāo)記抗體，洗去未結(jié)合的酶標(biāo)記抗體，加底物后顯色。若標(biāo)本中無相應(yīng)抗原，固相表面即無抗原結(jié)合，加入的酶標(biāo)記抗體則不能結(jié)合于固相并被洗滌去除，當(dāng)加入無色底物后，因無酶催化故不顯色。雙抗體夾心法適用于測定2價或2價以上的大分子抗原，但不適用于測定半抗原及小分子單價抗原，因其不能形成兩位點夾心。
(2)間接法：間接法是檢測抗體zui常用的方法，其原理為利用酶標(biāo)記的抗抗體以檢測已與固相結(jié)合的受檢抗體，故稱為間接法。先將待測的蛋白包被在孔板內(nèi)，然后依次加入一抗、酶標(biāo)記的二抗和底物顯色，通過儀器(例如酶標(biāo)儀)定量檢測抗原。這種方法操作簡單但由于高背景而特異性較差，目前已逐漸被夾心法取代。間接法的優(yōu)點是只要變換包被抗原就可利用同一酶標(biāo)二抗建立檢測相應(yīng)抗體的方法。間接法成功的關(guān)鍵在于抗原的純度。間接法中另一種干擾因素為正常血清中所含的高濃度的非特異性抗體。
(3)競爭法：競爭法可用于測定抗原，也可用于測定抗體。當(dāng)抗原材料中的干擾物質(zhì)不易除去，或不易得到足夠的純化抗原時，可用此法檢測特異性抗體。其原理為標(biāo)本中的抗體一定量的酶標(biāo)抗體競爭與固相抗原結(jié)合。標(biāo)本中抗體量越多，結(jié)合在固相上的酶標(biāo)抗體愈少，因此陽性反應(yīng)呈色淺于陰性反應(yīng)。如抗原為高純度的，可直接包被于固相。如抗原中有干擾物質(zhì)，直接包被不易成功，可采用捕獲包被法，即先包被與固相抗原相應(yīng)的抗體，然后加入抗原，形成固相抗原。洗滌除去抗原中的雜質(zhì)，然后再加入標(biāo)本和酶標(biāo)抗體進(jìn)行競爭結(jié)合反應(yīng)。小分子抗原或半抗原因缺乏可用于夾心法的兩個以上的位點，因此不能用雙抗體夾心法進(jìn)行測定，可以采用競爭法模式。其原理是標(biāo)本中的抗原和一定量的酶標(biāo)抗原競爭與固相抗體結(jié)合。標(biāo)本中抗原量含量愈多，結(jié)合在固相上的酶標(biāo)抗原愈少，zui后的顯色也愈淺。
7-木糖基-* "7-xylosyltaxol " X27155 "≥95% "
7-乙基-10-羥基* 7-Ethyl-10-Hydroxycamptothecin X27156 "≥98% "
7-乙基* 7-Ethylcamptothecin X27157 ≥98%
8-O-乙酰山梔苷甲酯 8-O-acetylshanzhiside methyl ester X27158 97%
8-甲氧基補(bǔ)骨脂素 8-Methoxypsoralen X27159 "≥98% "
8-姜酚 8-Gingerol X27160 "≥95% "
9-氨基* 9-Aminocamptothecin X27161 "≥98% "
Alpha-* Alpha-asarone X27162 "≥98% "
Apiosylskimmin Apiosylskimmin X27163 ≥98%
Menisdaurin Menisdaurin X27164 "≥98% "
Rosiridin Rosiridin X27165 "≥95% "
α-倒捻子素 α-Mangostin X27166 "≥98% "
β- 蛻皮甾酮 β-Ecdysone X27167 ≥99%
β-倒捻子素 β-Mangostin X27168 "≥98% "
"γ-日蟾毒配質(zhì) " γ-Bufotalin X27169 "≥98% "
阿馬堿 Ajmalicine X27170 ≥98%
安格洛苷C Angoroside C X27171 "≥98% "
安石榴苷 Punicalagin X27172 ≥97%
安五脂素 Anwuligan X27173 ≥98%
巴卡丁III Baccatin III X27174 "≥98% "
白花丹醌（白花丹素） plumbapin X27175 95%
表沒食子兒茶素(EGC) Epigallocatechin X27176 ≥98%
白花前胡丙素 Praeruptorin C X27177 "≥98% "
白花前胡丁素 Praeruptorin D X27178 "≥98% "
白花前胡素E Praeruptorin E X27179 "≥98% "
白樺脂酸 Betulinic acid X27180 "≥98% "
"白屈菜堿 " Chelidonine X27181 "≥98% "
白術(shù)內(nèi)酯 III Atractylenolide III X27182 "≥98% "
白術(shù)內(nèi)酯Ⅰ Atractylenolide I X27183 "≥98% "
"白頭翁素 " ANEMONIN X27184 "≥98% "
"白鮮堿 " Dictamnine X27185 "≥98% "
斑蝥素 Cantharidin X27186 ≥98%
"貝萼皂苷元 " Bayogenin X27187 "≥98% "
"扁塑藤素 " Pristimerin X27188 "≥98% "
扁蓄苷 Quercetin 3-O-alpha-L-arabinoside X27189 "≥98% "
表兒茶素沒食子酸酯 Epicatechin gallate X27190 "≥98% "
表沒食子兒茶素沒食子酸酯 EGCG X27191 "≥98% "
"檳榔堿 " Arecoline hydrobromide X27192 "≥98% "
補(bǔ)骨脂甲素 , 補(bǔ)骨脂二氫黃酮 Bavachin X27193 97%
補(bǔ)骨脂乙素，異補(bǔ)骨脂查爾酮 Isobavachalcone X27194 97%
蒼術(shù)苷A atractyloside A X27195 ≥97%
蒼術(shù)素 Atractylodin X27196 "≥97% "
藏紅花酸 Alpha-Crocetin X27197 "≥95% "
岑酮 Fraxinellone X27198 "≥98% "
柴胡皂苷C Saikosaponin C X27199 "≥97% "
"蟾毒靈 " Bufalin X27200 "≥98% "
蟾毒他靈 Bufotaline X27201 98%
"長春胺 " Vincamine X27202 "≥95% "
"長春地辛 " Vindesine X27203 "≥95% "
長春花堿 Vinblastine X27204 "≥98% "
"長春堿 " Vinblastine X27205 "≥95% "
* Vinpocetine X27206 ≥98%
長春新堿 Vincristine X27207 "≥92% "
長春質(zhì)堿 Catharanthine X27208 ≥95%
朝藿定A Epimedin A X27209 "≥98% "
朝藿定B Epimedin B X27210 "≥98% "
朝藿定C Epimedin C X27211 "≥98% "
翅子羅漢果I Siamenoside I X27212 "≥98% "
川陳皮素 Nobiletin X27213 "≥98% "
川芎嗪 Ligustrazine X27214 "≥98% "
"*內(nèi)酯,*乙素 " Andrographolide X27215 "≥98% "
*乙素 Andrographolide X27216 98%
"次百部堿 " Stemonidine X27217 ≥98%
刺五加苷B Eleutheroside B X27218 95%
"刺五加苷D " Eleutheroside D X27219 "≥98% "
"刺五加皂苷B " acanthopanax senticosides B X27220 "≥95% "
大豆皂苷AA Soyasaponin Aa X27221 "≥98% "
大豆皂苷BB Soyasaponin Bb X27222 "≥97% "
"大薊苷 " Pectolinarin X27223 "≥98% "
"大葉茜草素 " Mollugin X27224 "≥98% "
丹參酚酸A Salvianolic acid A X27225 ≥96%
丹參酮Ⅰ Tanshinone I X27226 "≥99% "
丹參酮ⅡA Tanshinone II A X27227 "≥98% "
丹酚酸C Salvianolic acid C X27228 98%
"* " Indirubin X27229 "≥98% "
"* " Eugenol X27230 "≥98% "
丁香樹脂醇雙葡萄糖苷 Syringaresinol-di-O-glucoside X27231 95%
丁香酸/3,5-二甲氧基-4-羥基苯甲酸 Syringic acid X27232 98%
*堿 Scopolamine butylbromide X27233 ≥98%
東莨菪堿 Hyoscin X27234 ≥98%
東莨菪內(nèi)酯/東莨菪素 Scopoletin X27235 ≥98%
冬綠苷 Gaultherin X27236 ≥98%
* Hiliedum X27237 97%
豆腐果新苷A Helicianeoide A X27238 "≥95% "
豆腐果新苷B Helicianeoide B X27239 "≥95% "
豆蔻明 Cardamonin X27240 ≥98%
"杜鵑素 " Farrerol X27241 "≥98% "
*皂苷A/醉魚草皂苷 Ⅳb Clinodiside A X27242 98%
對羥基苯甲酸乙酯 Ethyl p-hydroxybenzoate X27243 "≥98% "
對香豆酸 p-Coumaric acid X27244 "≥98% "
"對葉百部堿 " tuberostemonine X27245 ≥97%
多烯* Docetaxel X27246 "≥99% "
"莪二酮(莪術(shù)二酮/姜黃二酮) " Curdione X27247 "≥98% "
莪術(shù)醇 Curcumol X27248 "≥98% "
二氫丹參酮 I Dihydrotanshinone I X27249 "≥98% "
"二氫辣椒素 " Dihydrocapsaicin X27250 "≥98% "
二氫麻醉椒苦素 Dihidromethysticin X27251 "≥98% "
二氫石蒜堿 Dihydrolycorine X27252 "≥98% "
二氫楊梅素 Dihydromyricetin X27253 "≥98% "
二氫醉椒素 Dihydrokavain X27254 "≥99% "
番茄紅素 Lycopene X27255 "≥95% "
番瀉苷A Sennoside A X27256 "≥98% "
番瀉苷C Sennoside C X27257 "≥97% "
番瀉苷D Sennoside D X27258 "≥97% "
蜂斗菜內(nèi)酯 Bakkenolide X27259 ≥98%
佛手柑內(nèi)酯 Bergapten X27260 "≥95% "
"* " Glycine X27261 "≥98% "
甘草查爾酮A Licochalcone A X27262 "≥98% "
甘草苷元-7-O-D-芹糖-4’-O-D-葡萄糖苷 Liguiritigenin-7-O-D-apiosyl-4’-O-D-glucoside X27263 95%
甘草素 Liquiritigenin X27264 "≥97% "
甘草酸 Glycyrrhizic acid X27265 "≥98% "
*鹽 Glycyrrhizic acid ammonium salt X27266 "≥98% "
橄欖苦苷 Oleuropein X27267 "≥98% "
"高車前苷 " Homoplantaginin X27268 "≥95% "
高車前素 Hispidulin X27269 "≥98% "
高烏甲素 Lannaconitine X27270 ≥98%
藁本內(nèi)酯 Ligustilide X27271 ≥98%
根皮苷 Phlorizin dihydrateX27272 "≥97% "
根皮素 Phloretin X27273 "≥97% "
鉤藤堿 Rhynchophylline X27274 "≥97% "
鉤吻素子 Koumine X27275 ≥98%
枸橘苷 Poncirin X27276 "≥98% "
枸櫞酸血根堿 Sanguinarine citrate X27277 ≥98%
光甘草定 Glabridin X27278 "≥98% "
"桂皮醛 " Cinnamic aldehyde X27279 "≥98% "
"桂皮酸,肉桂酸 " Cinnamic acid X27280 "≥98% "
* d-Tetrandrine X27281 "≥97% "
